|
Abstract: Non cross-linked and cross-linked
collagen corneal shields pre-soaked in Gatifloxacin
sesquehydrate were studied for in vitro drug absorption.
Dissolution study and ex-vivo antimicrobial efficacy
testing against S. aureus was carried out. Drug content
in collagen corneal shield corresponded to its soaking
duration in drug solution. Collagen shields remained
intact and were successful in sustaining the drug. Drug
release depending on the degree of cross linking in the
collagen shields. Most of the drug was released within
0.5 - 4hr of dissolution of shield. Optimized
formulations were found to possess significant
antimicrobial efficacy. Appropriate dissolution duration
and antimicrobial efficacy of collagen corneal shields
propose them to be the potential useful drug carriers.
Introduction
Collagen shields are biologically inert, structurally
stable and biocompatible polymeric substances that may
be well used as drug delivery system. They have been
found to act as drug reservoir, which can deliver both
hydrophilic as well as hydrophobic drugs, increase the
contact time between drug and cornea as they can
reversibly bind drug molecules and release them in
delayed mode. Moreover, the cost of production of
corneal shields is low. Collagen shields are generally
manufactured from porcine or bovine collagen and three
different collagen shields are currently available with
dissolution times of 12, 24, and 72 hours. Presoaking
the collagen shield in a pharmacological agent
represents the most efficacious method of utilizing
collagen shields for drug delivery. They have been
observed to enhance drug delivery, promote epithelial
and stromal healing, neutralize collagenases, and reduce
corneal inflammation in different ophthalmic pathologies
like microbial keratitis, conjunctivitis and glaucoma
In the present study, a fourth generation
fluoroquinolone Gatifloxacin sesquehydrate (varying
concentrations) has been loaded on the Soft Shield® (a
marketed collagen corneal shield) with dissolution times
of 12-HOUR, 24-HOUR and 72-HOUR. The objective of the
study was to explore individual potential of these
shields to sustain the antibiotic release and to compare
the non cross-linked 12-HOUR shields with the
cross-linked 24-HOUR and 72-HOUR shields with reference
to in vitro drug release and ex vivo antimicrobial
efficacy.
For full text of this article contact the publisher on
info@kppub.com
|
The above content is an
abstract only. For the full Article please contact:
KONGPOSH Publications Pvt. Ltd.
ICS House, C-19, Commercial Complex, SDA, Opp. IIT Gate,
New Delhi, India -110016
Tel.: 26855839, 20057149, Fax: 91-11-26855876
Email:
info@kppub.com /
fpc@vsnl.com, Website:
http://www.kppub.com |
